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Published on 9 March 2020

****  ABACUS publications ****


Simultaneous extraction and purification of fucoxanthin from Tisochrysis lutea microalgae using compressed fluids. Journal of Separation Science.


Gallego R, Arena K, Dugo P, Mondello L, Ibáñez E, Herrero M. (2019)

Application of compressed fluids-based extraction and purification procedures to obtain astaxanthin-enriched extracts from Haematococcus pluvialis and characterization by comprehensive two-dimensional liquid chromatography coupled to mass spectrometry. Analytical and Bioanalytical Chemistry.​

Novoveská L, Ross M.E, Stanley M.S, Pradelles R, Wasiolek V and Sassi J-F. (2019)

Microalgal carotenoids: A review of production, current markets, regulations, and future direction. Marine Drugs, 17(11), 640.

Gallego R, Bueno M and Herrero M. (2019)

Sub- and supercritical fluid extraction of bioactive compounds form plants, food-by-products, seaweeds and microalgae - an update. Trends in analytical chemistry. 116, 198-213.

Gallego R, Martínez M, Cifuentes A, Ibáñez E and Herrero M. (2019)

Development of a green downstream process for the valorization of Porphyridium cruentum biomass. Molecules. 24 (8), 1564

Green extraction of bioactive compounds from microalgae. Journal of Analysis and Testing. 2 (2), 109–123.

****  ABACUS Consortium's background publications  ****


The Environmental Biorefinery: Using Microalgae to Remediate Wastewater, a Win-Win Paradigm. Delrue F, Alvarez-Diaz P, Fon-Sing S, Fleury G, Sassi JF. Energies 2016, 9, 132; doi:10.3390/en9030132

Using coagulation–flocculation to harvest Chlamydomonas reinhardtii: Coagulant and flocculant efficiencies, and reuse of the liquid phase as growth medium. F Delrue, Y Imbert, G Fleury, G Peltier, JF Sassi. Algal Research (2015), 9, 283-290

Comparison of pyrolysis and hydrothermal liquefaction of Chlamydomonas reinhardtii. Growth studies on the recovered hydrothermal aqueous phase. Hognon, C., F. Delrue, Texier, J., Grateau, M., Thiery, S., Miller, H., Roubaud, A.  Biomass and Bioenergy, (2015), 73(0): 23-31.

Marine Algae and the Global Food Industry. MH Abreu, R Pereira, JF Sassi. In « Marine Algae - Biodiversity, Taxonomy, Environmental Assessment, and Biotechnology » edited by L Pereira & J Magalhaes Neto. CRC Press (2014), pages 300 – 319.

Comparison of various microalgae liquid biofuel production pathways based on energetic, economic and environmental criteria. Delrue F, Li-Beisson Y, Setier P-A, Sahut C, Roubaud A, Froment AK, Peltier G. Bioresource technology (2013), 136, 205-212.

Preparation and characterization - including in situ Small Angle X-Ray Scattering - of gas chromatographic capillary columns with mesoporous silica thin films as stationary phases, D. Lefebvre, R. Rayes, J. El Sabahy, V. Jousseaume, F. Ricoul et al., J. Chromatography A, 1413 , (2015) , 85-93



Unlocking nature's treasure-chest: screening for oleaginous algae, Slocombe SP, Yi Zhang Q, Ross M, Anderson A, Thomas NJ, Lapressa A, Rad Menendez C, Campbell CN, Black KD, Stanley MS, Day JG (2015). (2015). Scientific Reports 5:9844 doi:10.1038/srep09844

Optimization of algal biomass and lipid production: effects of temperature and nutrient regimes on the batch culture of six oleaginous microalgae employing a two-phase cultivation strategy, Roleda MY, Slocombe SP, Bell E, Leakey R, Day JG, Stanley MS (2013). . Bioresource Technology 129: 439- 449

Blue Knowledge – Developing knowledge to support transition to a Blue Economy: A strategic approach, Greenhill L, Hughes A, Day JG, Stanley MS (2015)  Island Studies 3, 6-10.

Future algal biofuels: implications of environmental temperature on production strain selection, Day JG, Burt DJ, Achilles-Day UEM, Stanley MS (2015).  International Journal of Ambient Energy 36: 248- 252

Overcoming biological constraints to enable the exploitation of microalgae for biofuels,  Day, JG, et al (2012). Bioresource Technology 109: 245-251



Cell membrane permeabilization studies of Chlorella sp. by Pulsed Electric Fields, D. Rego, L. Costa, M. T. Pereira, L. M. Redondo, Member, IEEE. 2014.  EAPPC2014 – 5th Euro-Asian Pulsed Power Conference September 8-12, (2014), Kumamoto, Japan

Altered carbohydrate metabolism in glycogen synthase mutants of Synechococcus sp. strain PCC 7002: Cell factories for soluble sugars, Xu, Y. Guerra, LT et al. (2013).  Metab engineering, 16:56-67



Materials flow modeling of nutrient recycling in biodiesel production from microalgae. Rösch, C.; Skarka, J.; Wegerer, N. Bioresource Technology 107(2012), S. 191-199

Sustainability assessment of energy technologies: Towards an integrative framework, Grunwald, A.; Rösch, C.  Energy, sustainability and society 1(2011)3, open access DOI: 10.1186/2192-0567-1-3



Development of new Green processes for the recovery of bioactives from Phaeodactylum tricornutum, B. Gilbert-López, A. Barranco, M. Herrero, A. Cifuentes, E. Ibáñez (2017)  Food Research International. 99: 1056-1065.

Considerations in the use of enzyme-assisted extraction in combination with pressurized liquids to recover bioactive compounds from algae, Sánchez-Camargo, A.P., L. Montero, V. Stiger-Pouvreau, A. Tanniu, A. Cifuentes, M. Herrero, E. Ibáñez,. (2016) . Food Chemistry, 192: 67-74.

Downstream processing of Isochrysis galbana: A step towards microalgal biorefinery, B. Gilbert-López, J.A. Mendiola, J. Fontecha, L.A. M. van den Broek, L. Sijtsma, A. Cifuentes, M. Herrero, E. Ibáñez (2015). Green Chemistry. 17, 4599-4609.

Green processes and sustainability: an overview on the extraction of high added-value products from seaweeds and microalgae, M. Herrero, E. Ibáñez (2015). Journal of Supercritical fluids, 96: 211-216.

Separation and characterization of phlorotannins from brown algae Cystoseira abies-marina by comprehensive two-dimensional liquid chromatography, L. Montero, M. Herrero, E. Ibáñez, A. Cifuentes (2014). Electrophoresis, 35: 1644-1651.



  1. Patents (EP 1 169 428 B1 and EP 1 326959 B1): Protected PBR technology, manufacturing process
  2. Patent (WO 2017055585 A1): Protected PBR operating strategy reducing operational expenditures

Repeated fed-batch cultivation of Thermosynechococcus elongatus BP-1 in flat-panel airlift photobioreactors with static mixers for improved light utilization: influence of nitrate, carbon supply and photobioreactor design. Bergmann, P.; Trösch, W.  Algal Research-biomass biofuels and bioproducts, (2016)17, 79-86



Ryabova L., Guillemer S., Pallas S., Persillon C., Lefèvre F., Masson J.M., Ravot G. A quick in vitro pathway from prokaryotic genomic libraries to enzyme discovery. Biotechniques. (2008) Jul;45(1):63-8.